Abstract
Background and objectivesColon cancer is a frequently occurring primary malignant tumor. Chemotherapy can reduce the risk of local and distant relapse. Therefore, it is very important to find new biomarkers that can predict chemoresistance and help in individuate treatment decision.Design and setting Retrospective analysis of 126 patients, who were treated at our department between June 2010 and December 2014.Patients and methodsIn this study, we examined the expression levels of 1200 human miRNAs in colon cancer tissues, using laser capture microdissection and microRNA profiling arrays. A validation study was done to corroborate a subset of the results, including expression levels of miR-4299, miR-196b, miR-324-5p, miR-455-3p and miR-939, through analyzing stage IV colon adenocarcinoma tissues (not responding and responding to the chemotherapy) with laser capture microdissection and quantitative real-time PCR. We analyze the relationship between the expression levels of these miRNAs and the survival rate of colon cancer patients by Kaplan–Meier method.ResultsWe found that miR-4299 and -196b have significant diagnostic value in chemoresistant colon cancer. MiR-4299 yielded an AUC (the areas under the ROC curve) of 0.810 and miR-196b yielded an AUC of 0.726 in discriminating chemoresistant colon cancer from controls. Combined with ROC analyses of these 2 miRNAs revealed an elevated AUC of 0.877 with 71.4 % sensitivity and 95.5 % specificity in discriminating chemoresistant colon cancer. The low level of miR-4299 expression and the high level of -196b expression are significantly correlated with better survival of colon cancer patients.DiscussionThese data suggest that miR-4299 and -196b have strong potential as novel biomarkers for chemoresistance detection of colon cancer.
Highlights
Background and objectivesColon cancer is a frequently occurring primary malignant tumor
These data suggest that miR-4299 and -196b have strong potential as novel biomarkers for chemoresistance detection of colon cancer
Expression profiles of miRNAs correlated to colon cancer chemoresistance To investigate the roles of miRNA in colon cancer chemoresistance, we first examined the miRNA expression profiles with Agilent Human MicroRNA Array (Among the 1367 unique miRNA probes) on 4 responder colon cancer and 4 non-responder colon cancer to the chemotherapy
Summary
Colon cancer is a frequently occurring primary malignant tumor. Due to the poor prognosis and distant invasion and migration, the overall incidence of colon cancer is approximately 5 % and the 5-year survival rate of colon cancer patients is very low [2]. In China, the morbidity and mortality of MicroRNAs (miRNAs) are small, noncoding sequences, approximately 18–25 nucleotides long, which are post-transcriptional regulators of gene expression. They use imperfect base-pairing with the 3′-untranslated region (3′ UTR) of messenger (m) RNA, usually resulting in translational repression and gene silencing. Depending on the genes miRNAs are regulated; miRNAs can function as either oncogenes or tumor suppressors [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
