Abstract

Specific metal deposition on metal nanoparticles plays an important role in molecular construction as well as for microscopic visualization and signal enhancement in various bioanalytical methods. To characterize this process we studied surface‐immobilized gold nanoparticle ensembles at single particle level by atomic force microscopy monitoring the very same particle arrangements after each enhancement step. Thereby, enhancement solutions of different composition are studied regarding their enhancement rate as measured by the growing height of the particles at single particle level. Other features of interest were the influence of the diameter of the seed particle on the growth process and the influence of exchange of enhancement solution. For the applied conditions (low nanoparticle surface concentration subjected to fresh enhancement solution 2 min each) we observed a linear growth regime, which was dependent on the particles’ seed diameter and differed considerably in enhancement rate, homogeneity, and specificity among the examined enhancement solutions. We extended these studies to an investigation of enzymatic metal deposition where an enzyme complex (horseradish peroxidase) catalyzes the growth of a metal layer.

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