Abstract
Human urinary mucopolysaccharides (MPS) were isolated and identified by a technique involving cetyltrimethylammonium bromide (CTAB) precipitation, cellulose acetate electrophoresis and enzymic hydrolysis with testicular hyaluronidase, chondroitinase AC and chondroitinase ABC. The MPS preparations contain no sialic acid or phosphate and thus appeared to be free of glycoproteins and nucleoproteins. The uronic acid: hexosamine ratios range from 0.8 to 1.5. In children and adults the main urinary MPS are chondroitin-4-sulfate and/or chondroitin-6-sulfate with heparan sulfate as a minor component. Little or no dermatan sulfate, hyaluronic acid, heparin or keratan sulfate was found. Urinary MPS of children contain large amounts of a hyaluronidase-resistant, chondroitinase AC-susceptible compound whereas in adults this fraction is greatly diminished or absent. Children with a “marfanoid” syndrome gave positive CTAB screening tests but were within normal limits of MPS excretion when expressed in μmole/mmole creatinine, μmoles/1, μmoles/24 h and nmoles/24 h/kg body weight.
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