Abstract

Objective The objective of this study was to analyze the immune effects of pVR-S and rAdV-S with different immune strategies. Methods HBsAg gene was used to construct recombinant DNA plasmid named pVR-S and recombinant adenovirus named rAdV-S, respectively. Firstly, BALB/c mice were injected with pVR-S by two methods, including electroporation and intramuscular injection. Secondly, two groups of mice were immunized with rAdV-S and pVR-S, respectively. Level of HBsAb was detected by ELISA and cellular responses were detected by Elispot to evaluate the immune effects. Finally, DNA prime-Adenovirus boost strategy was used to evaluate the humoral and cellular immune responses. Results Compared with intramuscular immunization, electroporation-mediated immunization could induce stronger humoral immune response. Independent pVR-S increased HBsAb level slowly, while independent rAdV-S induced HBsAb at week 2 with higher HBsAb level than pVR-S. What is more, pVR-S prime-rAdV-S boost strategy could result in two to three times higher cellular responses than independent immunizations. Conclusion Recombinant adenovirus rAdV-S could induce strong humoral and cellular immune responses. pVR-S prime-rAdV-S boost immunization was better than independent rAdV-S immunization, contributing to excellent antiviral capability. Key words: Hepatiis B surface antigens; DNA; Adenoviridae; Immunization, secondary

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