Abstract
Human marrow mononuclear (marrow) cells from normal donors who had not recently received tetanus toxoid (TT) booster immunizations were examined for their ability to produce specific IgG anti-TT antibody (anti-TT), polyclonal IgG and polyclonal IgM with and without in vitro tetanus toxoid (TT) stimulation. ELISA assays were used to measure specific and polyclonal IgG and IgM production. Marrow B cells were found to secrete anti-TT and nonspecific IgG and IgM spontaneously for up to 21 days of culture. Stimulation of arrow cultures with TT resulted in variable modulation of anti-TT production, yet had no detectable effect on polyclonal IgG or IgM production. Depletion of T cells from narrow caused a marked reduction of spontaneous and TT-induoed anti-TT synthesis. Repletion of T cell-depleted marrow with autologous peripheral blood T cells and stimulation with TT were required to reconstitute anti-TT production. The results show that: 1) steady state marrow contains partially activated or differentiated marrow B cells capable of producing in vitro anti-TT; 2) spontaneous anti-TT synthesis by marrow B cells is regulated, in part, by T cells. Since antigen and T cells can alter the function of arrow B cells, these findings suggest that antigen-specific immune memory directed at pathogens or tunor antigens may be manipulable before, during, or after bone marrow transplantation.
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