Abstract
Methods b-Lactoglobulin, whole casein and its four different fractions, i.e. as1-, as2-, band -caseins, were isolated from raw CM and GM. Purified b-caseins were subjected to a mild proteolysis by plasmin which generated 3 peptides, i.e. f(1-28), f(29-107) and f(108-207/9). Synthetic peptides partially recovering the N-terminal f(29107) part of the caprine b-casein were also produced. Immunoreactivity of the purified proteins and peptides was assessed by IgE binding studies using sera from 12 GM-allergic patients tolerant to CM and 10 CM-allergic patients. The capacity of bovine and caprine milk proteins to induce the degranulation of humanized rat mast cells passively sensitized with human specific IgE was also evaluated.
Highlights
Allergy to goat’s milk (GM) proteins in patients tolerant to cow’s milk (CM) is nowadays often observed whereas CM allergy was generally associated with a cross allergy to GM
We aimed to analyse the specific IgE response in patients allergic to GM but tolerant to CM and to compare this response to that observed in patients allergic to both milks
Allergy to GM in patients tolerant to CM is associated with an IgE response specific to caprine caseins without any cross reactivity to bovine counterparts despite sequence homology of ca. 90%
Summary
Allergy to goat’s milk (GM) proteins in patients tolerant to cow’s milk (CM) is nowadays often observed whereas CM allergy was generally associated with a cross allergy to GM. Specific IgE responses in patients allergic to goat’s milk but tolerant to cow’s milk: involvement of minor differences in primary structure between caprine and bovine caseins From Food Allergy and Anaphylaxis Meeting 2011 Venice, Italy. Background Allergy to goat’s milk (GM) proteins in patients tolerant to cow’s milk (CM) is nowadays often observed whereas CM allergy was generally associated with a cross allergy to GM.
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