Abstract
Inosine monophosphate (IMP) is a key factor affecting the fleshy flavor of meat; meanwhile, the free-range mode is an efficient strategy to improve muscular IMP content. To assess expression differences in IMP metabolism-related genes under different feeding patterns, Illumina Nextseq 500 sequencing was used to catalog the global gene expression profiles of muscle samples from Lueyang black-bone chicken under free-range and caging conditions. A total of 15510 unigenes were assembled, with 13423 (86.54%) and 6088 (39.25%) unigenes correctly annotated in the GO and KOG databases, respectively. Next, the "purine metabolism" pathway in the "nucleotide metabolism group" was assessed in depth. Through Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, we retrieved 172 nucleotide- and 5 purine- metabolism related genes that were differentially expressed in muscle samples from free-range and caged chickens. At 60-day-old, AMPD1, NT5C1A and ENTPD8 showed higher levels in the free-range group, while only ENTPD8 was upregulated in 120-day-old chickens. In addition, GART, GARS and ADSL in free-range chickens showed higher levels compared with caged animals. Furthermore, IMPDH levels in free-range chicken were lower than those of caged chicken. Real-time quantitative polymerase chain reaction (qPCR) was used to validate the above findings. These results revealed a set of differentially expressed genes potentially related to IMP metabolism in chicken under different breeding modes, providing novel insights into controlling IMP levels in chicken meat.
Highlights
In the past few decades, scientists have mainly focused on the appearance, growth performance and feed reward of broiler chickens, and the growth rate and muscle production yields of chicken have been greatly improved
In order to evaluate the flavor quality of chicken meat under different feeding patterns, Inosine monophosphate (IMP) contents in breast and thigh muscle samples from 60- and 120-day-old animals were detected by high performance liquid chromatography (HPLC), respectively
The results showed that 2 additional genes (GART and ADSL) were significantly up-regulated in the free-range group compared with caged animals
Summary
In the past few decades, scientists have mainly focused on the appearance, growth performance and feed reward of broiler chickens, and the growth rate and muscle production yields of chicken have been greatly improved. It is unfortunate that the quality of chicken meat decreases significantly with increasing growth rate. It remains challenging to improve chicken meat quality while maintaining a high growth rate. Wholesalers have acknowledged delicate flavor as an important indicator of meat quality [1].
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