Abstract
The results presented demonstrate the expression of pfkfb4 gene in adult testis and in a mouse spermatogonia germ cell line (GC-1spg). The genomic organization of the human pfkfb4 gene shows the existence of 14 exons and 13 introns, spanning 45 kb. A detailed analysis of the 5′-flanking region by transient transfection assays with different 5′-deletion promoter constructs in GC-1spg and mouse sertoli cells (TM-4), allows us to define the minimal promoter unit, containing several GC-rich and ETF sequences along the first −141 nucleotides involved in basal expression. This gene is activated by serum and chemical hypoxia (CoCl2 treatment) whereas β-estradiol decreases its expression.
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