Specific detection of acetamiprid with aptamer based on flexible and adhesive SERS membrane

Abstract

Acetamiprid poses a serious threat to human beings. In this work, the highly transparent fluorescent quantitative PCR sealing membranes (PCR-M) developed in our previous work which had attractive characteristics such as sensitivity, stability and reproducibility, and its capability of extracting analytes quickly, was used to detect acetamiprid. The detection limit of 1 nmol/L was obtained by direct detection of CN group in acetamiprid. In order to improve the selectivity and sensitivity, acetamiprid aptamer was introduced. After the interaction of aptamer with acetamiprid the SERS signal of adenine at 730 cm−1 increased. The detection limit of indirect detection was 10-8 mol/L. Aptamer advanced Ag-PCR-M-based Surface enhanced Raman spectroscopy (SERS) method for indirect SERS detection which significantly improves the detection sensitivity and greatly increases the range of signal response value. This will be conducive to the detection of actual samples and expand the practical application.