Abstract
BackgroundClassic goose parvovirus (cGPV) causes high mortality and morbidity in goslings and Muscovy ducklings. Novel GPV (N-GPV) causes short beak and dwarfism syndrome (SBDS) in Cherry Valley ducks, Pekin ducks and Mule ducks. Both cGPV and N-GPV have relatively strict host specificity, with obvious differences in pathogenicity. Specific detection of cGPV and N-GPV may result in false positives due to high nucleotide similarity with Muscovy duck parvovirus (MDPV). The aim of this study was to develop a highly specific, sensitive, and reliable TaqMan real-time PCR (TaqMan qPCR) assay for facilitating the molecular detection of cGPV and N-GPV.ResultsAfter genetic comparison, the specific conserved region (located on the NS gene) of cGPV and N-GPV was selected for primer and probe design. The selected regions were significantly different from MDPV. Through a series of optimization experiments, the limit of detection was 50.2 copies/μl. The assay was highly specific for the detection of cGPV and N-GPV and no cross-reactivity was observed with E. coli., P.M., R.A., S.S., MDPV, N-MDPV, DAdV-A, DEV, GHPV, DHAV-1, DHAV-3, ATmV, AIV, MDRV and N-DRV. The assay was reproducible with an intra-assay and inter-assay variability of less than 2.37%. Combined with host specificity, the developed TaqMan qPCR can be used for cGPV and N-GPV in differential diagnoses. The frequency of cGPV in Muscovy duckling and goslings was determined to be 12 to 44%, while N-GPV frequency in Mule ducks and Cherry Valley ducks was 36 to 56%. Additionally, fluorescence-positive signals can be found in Mule duck embryos and newly hatched Mule ducklings. These findings provide evidence of possible vertical transmission of N-GPV from breeding Mule ducks to ducklings.ConclusionsWe established a quantitative platform for epidemiological investigations and pathogenesis studies of cGPV and N-GPV DNA that was highly sensitive, specific, and reproducible. N-GPV and cGPV infections can be distinguished based on host specificity.
Highlights
Classic goose parvovirus causes high mortality and morbidity in goslings and Muscovy ducklings
Mark the variation GenBank accession numbers of Goose parvovirus (GPV) strains B2The GenBank accession numbers of GPVs exclude MF441222 B3The GenBank accession numbers of GPVs exclude KU684472 B4The GenBank accession numbers of GPVs exclude EU583391, EU583389, KT343253 and MF441226 C1 Muscovy duck parvovirus (MDPV) sequence compared with the GPV-qP, the variations are mark with square box, the results showed that the designed GPV-qP is specificity MDPV D1 The GenBank accession numbers of MDPV: U22967 and X75093 MDPVD2The GenBank accession numbers of MDPV: KU844282, KU844281, KT865605, KX000918, JF926697, KC171936, JF926698 and KY744743 MDPVD3The GenBank accession numbers of MDPV: KY069274, JF926695, KY511293 and JF926696
We demonstrated that Novel GPV (N-GPV) appeared to possible vertically transfer from breeder Mule ducks to ducklings
Summary
Classic goose parvovirus (cGPV) causes high mortality and morbidity in goslings and Muscovy ducklings. Waterfowl parvoviruses, including goose parvoviruses (GPVs), Muscovy duck parvoviruses (MDPVs) and the variant viruses of GPVs and MDPVs, were renamed as Anseriform dependoparvovirus 1 by the International Committee on Taxonomy of Viruses (ICTV) and have been assigned to the genus Dependoparvovirus in subfamily Parvovirinae under family Parvoviridae based on similarities in phylogenetic properties (https://talk.ictvonline.org/taxonomy/). These viruses contain a linear, single-stranded DNA genome (approximately 5.1 kb in length). The VP2 and VP3 contain the same carboxyl-terminal portion as VP1 in these viruses [1,2,3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
