Specific coaggregation and the cell wall of Streptococcus sanguis.

Abstract

Sacculi prepared from Streptococcus sanguis 34 by extensive extraction of cells with hot sodium dodecyl sulfate-2-mercaptoethanol retained the ability to coaggregate with Actinomyces viscosus T14V. When S. sanguis 34 was disrupted by homogenization with glass beads and fractionated by differential centrifugation, only the cell wall fraction agglutinated A. viscosus T14V. When strain 34 was treated with lysozyme, the coaggregating capability of the cells was essentially unaltered. Sacculi prepared from lysozyme-treated strain 34 and additionally purified by electrophoresis were agglutinated by strain T14V.