Specific and sensitive assay method for vasopressin and oxytocin using glass-paper chromatography.

Abstract

ALTHOUGH publications concerning measurement of vasopressin and oxytocin in blood and urine are legion, the lack of specificity of technique for concentration and bioassay of the trace amounts presumed to be present in biological fluids has led to wide variation in results and frank scepticism concerning their physiological significance1. Since bioassay techniques, although extremely sensitive, are inherently subject to wide error, a reliable quantitative method applicable to biological fluids demands a specific and sensitive technique for isolation and concentration of these polypeptides from blood or urine prior to bioassay.