Specific and quantitative detection of bacteria based on surface cell imprinted SERS mapping platform

Abstract

Non-specificity and poor quantitative ability are the main challenges in surface-enhanced Raman scattering (SERS) technique, especially for the detection of bacteria in real samples. In this study, we presented a surface cell imprinted SERS mapping platform which is competent for the specific and quantitative detection of bacteria. The platform based on the fabrication of a surface cell imprinted substrate (SCIS) by which Escherichia coli (E. coli) can be captured and labelled by SERS tags which produces strong characteristic signal to indicate the capture of targets. We highlighted the specificity of this platform in the detection of E. coli, by comparing the performances toward Salmonella paratyphoid A, Bacillus subtilis, Enterococcus faecalis and Staphylococcus aureus. Upon integrating with SERS mapping technique, the platform displayed good quantitative ability toward E. coli with a wide linear range from 102 to 108 CFU/mL and a low detection limit of ∼1.35 CFU/mL. Moreover, this novel SERS analysis platform was proved to be effective for E. coli detection in real probiotic beverage and chicken breast meat samples. By fabricating different SCISs, this platform can be replicated for the detection of other bacteria, which provides a promising application for real sample testing.