Species-specific identification of adulteration in cooked mutton Rista (a Kashmiri Wazwan cuisine product) with beef and buffalo meat through multiplex polymerase chain reaction.

M. Mansoor Bhat,Sheikh Adil,M. Ashraf Pal,Imtiyaz A. Mantoo,Mir Salahuddin,Henna Jalal

doi:10.14202/vetworld.2016.226-230

M. Mansoor Bhat, Sheikh Adil + Show 4 more

Open Access

https://doi.org/10.14202/vetworld.2016.226-230

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Abstract

Aim:Meat adulteration is a serious problem in the meat industry and needs to be tackled to ensure the authenticity of meat products and protect the consumers from being the victims. In view of such likely problem in indigenous meat products of Kashmiri cuisine (Wazwan), the present work was performed to study the detection of beef and buffalo meat in cooked mutton Rista by mitochondrial DNA (mtDNA) based multiplex polymerase chain reaction (PCR) method under laboratory conditions.Materials and Methods:Three experimental trials were conducted wherein the products were prepared from pure mutton, beef and buffalo meat, and their admixtures in the ratios of 60:20:20, 80:10:10, 90:05:05 and 98:01:01, respectively.Results:The primers used in the study amplified the cyt b gene fragments of sizes 124 bp, 472 bp and 585 bp for buffalo, cattle and sheep, respectively. It was possible to detect cattle and buffalo meat at the level of 1% in the mixed meat cooked Rista. The multiplex PCR successfully amplified cyt b gene fragments of mtDNA of the target species and thus produced characteristic band pattern for each species. The band intensities of cattle and buffalo in the mixed meat Rista progressively decreased corresponding to their decreasing level from 20% to 1%. Processing, cooking (moist heating) and non-meat formulation ingredients had no effect on detection of meat species adulteration.Conclusion:The multiplex PCR procedure standardized and developed in this study is simple, efficient, sensitive, reliable and highly specific for detecting falsification of cooked mutton product with beef and buffalo meat up to 1% level.

Highlights

The first case of fraudulent substitution was recorded in 13th century A.D. at Florence in Italy [1], and this fraudulent adulteration of costly meat with cheap meat is a common practice observed throughout the world
polymerase chain reaction (PCR) analysis of species-specific mitochondrial DNA sequences is the most common method currently used for identification of meat species in food [7,8,9,10,11,12] and animal feedstuffs [13,14,15]
The band intensities for the cattle and buffalo cyt b gene fragments showed a progressive decrease with the decrease of their proportion from 20% to 1% in the cooked mutton Rista

Summary

Introduction

The first case of fraudulent substitution was recorded in 13th century A.D. at Florence in Italy [1], and this fraudulent adulteration of costly meat with cheap meat is a common practice observed throughout the world. PCR analysis of species-specific mitochondrial DNA (mtDNA) sequences is the most common method currently used for identification of meat species in food [7,8,9,10,11,12] and animal feedstuffs [13,14,15]. The PCR assays have been employed for the identification of species origin of meat and meat products targeting genomic and mtDNA [16,17,18,19]. Species-specific PCR assay was developed for quick and authentic identification of chicken, beef and buffalo meat, even in heat processed admixed meat products containing the non-targeted species [21]

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