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Abstract
The jelly coat surrounding the eggs of amphibia is composed of oviducal mucins and plays an important role in the fertilization process. From a structural and chemical point of view, these jellies are very different from one species to another. Bufo viridis is the 13th amphibia species studied in term of carbohydrate structural analysis. The oligosaccharides have been released from the oviducal mucins by reductive β elimination, purified by various chromatography procedures and analyzed by 1H and 13C 1D–2D NMR spectroscopy. Among the 15 compounds, ten have novel structures, although they possess some well-known structural patterns as blood group epitopes (Le x, Le y) or other sequences already observed in other amphibia species. These results reinforce our hypothesis about the strict species-specificity of these carbohydrate chains. It must be noted that such species-specificity does not depend on one particular monosaccharide but it is rather due to a set of particular tri- or tetrasaccharide sequences. Hence, B. viridis species could be characterized by the simultaneous presence of a 2,3,6-trisubstituted galactosyl residue, the GlcNAc(β1–3)[Fuc(α1–4)]GlcNAcβ sequence and the Le x, Le y or Cad determinants. The anionic charge of the oligosaccharides is carried only by sialic acid α-(2→6)-linked to GalNAc-ol residue as in Bufo bufo or in Bufo arenarum.
Published Version
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