Species-specific PCR primers for detection of Xanthomonas vesicatoria

Abstract

This study presents the development of a PCR method for specific detection of Xanthomonas vesicatoria, causal agent of bacterial spot of tomato and pepper. Primer pair XV1F and XV1R was designed based on partial DNA sequence of the catalytic subunit of the ATP synthase gene atpD and tested using a collection of various bacterial pathogens mainly for tomato and pepper. After optimization of the PCR conditions, the 365 bp long fragment was only produced in X. vesicatoria samples. Therefore, the primers and protocol allow rapid and species-specific detection of X. vesicatoria.