Abstract

Simple SummaryNocturnal fireflies are facing various challenges to their survival (e.g., artificial light pollution and extreme climate change). However, there are still no effective diagnostic tools to distinguish firefly species and monitor how their population size and nocturnal behavior change in the wild. Here, we demonstrated that flash interval (FI), a timing character of firefly flash signaling, can be a reliable species-specific luminescent marker for identifying three species (Abscondita cerata, Luciola kagiana, and Luciola curtithorax) in a wild sympatric population and evaluating their nightly behaviors in video-imagery analysis, as well as a traditional field specimen collection. Our studies provide key information and valuable references for future studies of (1) the behaviors of sympatric fireflies in the wild and (2) the development of automatic and rapid digital diagnostic tools for identifying firefly species and quantifying population size, especially for firefly conservation.It is highly challenging to evaluate the species’ content and behavior changes in wild fireflies, especially for a sympatric population. Here, the flash interval (FI) and flash duration (FD) of flying males from three sympatric species (Abscondita cerata, Luciola kagiana, and Luciola curtithorax) were investigated for their potentials in assessing species composition and nocturnal behaviors during the A. cerata mating season. Both FI and FD were quantified from the continuous flashes of adult fireflies (lasting 5–30 s) via spatiotemporal analyses of video recorded along the Genliao hiking trail in Taipei, Taiwan. Compared to FD patterns and flash colors, FI patterns exhibited the highest species specificity, making them a suitable reference for differentiating firefly species. Through the case study of a massive occurrence of A. cerata (21 April 2018), the species contents (~85% of the flying population) and active periods of a sympatric population comprising A. cerata and L. kagiana were successfully evaluated by FI pattern matching, as well as field specimen collections. Our study suggests that FI patterns may be a reliable species-specific luminous marker for monitoring the behavioral changes in a sympatric firefly population in the field, and has implication values for firefly conservation.

Highlights

  • Among the various glowing organisms, fireflies (Coleoptera: Lampyridae) are one of the best-known bioluminescent creatures due to their amazing flash communications during courtship [1]

  • The adult light organ can generate the light into discrete rapid flashes, which are controlled by nitric oxide (NO) released from the nerve cells via oxygen-gating to photocytes [4,5]

  • We further investigated whether any species-specific luminous marker can be applied to assess the nightly changes in species compositions and flash behaviors of a wild sympatric population during A. cerata massive occurrence

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Summary

Introduction

Among the various glowing organisms (e.g., bacteria, jellyfish, fungi), fireflies (Coleoptera: Lampyridae) are one of the best-known bioluminescent creatures due to their amazing flash communications during courtship [1]. Adult fireflies emit light via a specialized abdominal light organ where luciferase–luciferindependent luminescent reaction occurs [4]. The adult light organ can generate the light into discrete rapid flashes, which are controlled by nitric oxide (NO) released from the nerve cells via oxygen-gating to photocytes [4,5]. Fireflies’ luminous courtships have been intensively studied for decades [3,9], their nightly behaviors remain a mystery. Do they glow or flash throughout the entire night? Do they glow or flash throughout the entire night? Do they mate during a specific time of night? How do distinct firefly species cohabit without interfering with each other’s luminous communications?

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