Species Identification of Dermatophytes by MALDI-TOF MS

Abstract

MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry (MS) is a new tool for the identification of microorganisms, inclusive of dermatophytes. The technique is faster, more straightforward, and powerful when compared to conventional dermatophyte identification methods (culture and microscopy). Accurate species identification in dermatophytes is not only essential to survey epidemiologic situations, but also for an appropriate medical treatment and to locate the source of infection (zoophilic or anthropophilic). Multiple platforms from a number of well-established commercial manufacturers (Andromas SAS, Bruker Daltonics, bioMerieux) have been used for dermatophyte identification with different success. Independent from the platform used, all of the studies reviewed here report on problems with the identification of phylogenetically closely related anthropophilic and zoophilic dermatophyte species. Thus, supplementation of the databases/libraries as well as standardized extraction protocols and cultivation methods are the precondition required for optimal species identification.