Abstract
A method was standardized to isolate quality DNA from cattle and buffalo fat for species identification using QIAamp DNA stool mini kit. The quality of the DNA was sufficient enough to amplify universal primers viz., mt 12S rRNA and mt 16S rRNA, and species specific D loop primers for cattle and buffalo. The sensitivity of the PCR assay in the species specific D loop primer amplification was with a detection level of 0. 47ng cattle DNA and 0.23ng buffalo DNA in simplex and, 0. 47ng cattle DNA and 0.12ng buffalo DNA in duplex PCR. It is a potentially reliable method for DNA detection to authenticate animal fat.
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