Species distinctness of Hauffenia michleri (Kuščer, 1932) (Caenogastropoda: Truncatelloidea: Hydrobiidae)

Andrzej Falniowski,Magdalena Szarowska

doi:10.12657/folmal.023.016

Abstract

Hauffenia michleri (Kuscer, 1932) was described from Mocilnik spring in Slovenia. Later it was regarded as conspecific with H. tellinii Pollonera, 1898, since no differences in the soft parts morphology and anatomy were found. In the present paper, applying molecular markers (mitochondrial cytochrome oxidase subunit I and nuclear 18S ribosomal RNA partial gene sequences), the genetic differences typical of congeneric distinct species (p distances 0.067, and 0.005, respectively) were found, proving the species distinctness of H. michleri. key words: mtDNA, COI, 18SrRNA, p-distance, Slovenia

Highlights

Močilnik, the main source of the Ljubljanica River, Slovenia is inhabited by interesting representatives of the Hydrobiidae (Bole 1967, 1985, Radoman 1983)
The tissue was hydrated in TE buffer (3 × 10 min.); total genomic DNA was extracted with the SHERLOCK extracting kit (A&A Biotechnology), and the final product was dissolved in 20 μl TE buffer
The PCR reaction was performed with the following primers: LCO1490 ( 5 ’ - GGTCAACAA AT C ATA A A G ATAT T G G - 3 ’ ) (Folmer et al 1994) and COR722b (5’-TAAACTTCAGGGTGACCAAAAAATYA-3’) (Wilke & Davis 2000) for the mitochondrial cytochrome oxidase subunit I (COI) gene and SWAM18SF1 (5’-GAATGGCTCATTAAATCAGTCGAGGTTCCTTAGATGATCCAAATC-3’), and SWAM18SR1 ( 5 ’ -AT CCTCGT TA AAGGGTT TA A AT TA AAGTG GTGTACTCATTCCAATTACGGAGC-3’) for the nuclear 18S ribosomal RNA (18S) gene (Attwood et al 2003)

Summary

Introduction

The main source of the Ljubljanica River, Slovenia is inhabited by interesting representatives of the Hydrobiidae (Bole 1967, 1985, Radoman 1983). Kuščer (1932) described a minute valvatiform hydrobiid snail Hauffenia michleri (Fig. 1) from this locality. Later, considering the shell and anatomy, Bodon et al (2001) synonymised H. michleri (Kuščer, 1932) with H. tellinii Pollonera, 1898 from

Results

Conclusion