Abstract
The rDNA ITS barcoding marker has been widely used for plant identification, along with other primers for the chloroplast sequence region. This study applied the ITS primer to discriminate Tristaniopsis spp. on Bangka Island. Twelve leaf samples were collected from five community forests in Bangka district. The pairwise distance between sequences calculated using Kimura’s two parameters in MEGA11 revealed that pelawan air is different from other pelawan specimens (1.58–2.1%), while pelawan orange and pelawan merah specimens showed low variation (0–0.78%). There is a barcoding gap in the application of the ITS markers (full-length ITS including 5.8s, ITS1, and ITS2) for Tristaniopsis, which is shown by the higher value of minimum interspecific divergence than the value of maximum interspecific divergence for all three regions. Phylogeny trees constructed from the nucleotide sequence of 12 pelawan and other related taxa clustered pelawan merah and pelawan orange together and separated them from pelawan air.
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