Abstract
Our previous study demonstrated that there are species differences among vertebrates in their conversion of 7α-hydroxycholesterol (7HC) to 7-ketocholesterol (7KC). To examine this further, we investigated the differences in the products of 7α-hydroxycholesterol in various species of male muroid rodents. Adult male Syrian hamsters ( Mesocricetus auratus), dwarf hamsters ( Phodopus rovolovskii), Djungarian hamsters ( Phodopus sungorus), Chinese hamsters ( Cricetulus griseus), rat-like hamsters ( Tscherskia triton), and hispid cotton rats ( Sigmodon hispidus) were used. Microsomal fractions were prepared from their livers, and the activities of the enzymes that participate in the dehydrogenation of 7α-hydroxycholesterol were determined by measuring the products using high-performance liquid chromatography. 7α-hydroxycholesterol was converted to both 7α-hydroxy-4-cholesten-3-one (7HCO) and 7-ketocholesterol in all of the hamsters tested. However, in the rat-like hamster and the hispid cotton rat, 7α-hydroxycholesterol was converted mostly to 7α-hydroxy-4-cholesten-3-one, as also observed in the rat ( Rattus norvegicus). The results suggest that microsomal enzyme activity in the conversion of 7α-hydroxycholesterol to 7-ketocholesterol varies considerably, even within the subfamily Cricetinae and the family Muridae.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
