Abstract

On the basis of molecular genetic analysis of the intron-1 of the nuclear serum albumin gene (SAI-1) were identified 177 individuals of Pelophylax esculentus complex of 9 localities Belgorod. Two types of population systems R and RE were identified. Pure populations of L-type, E-type and LE-type as well as P. lessonae individuals were not identified.

Highlights

  • The European green frogs complex (Pelophylax esculentus complex) includes three species: Pelophylax ridibundus (Pallas, 1771), P. lessonae (Camerano, 1882) and P. esculentus (Linnaeus, 1758) [1]

  • More precise molecular genetic methods should be used to reliably identify the species belonging to Pelophylax esculentus complex [2, 3]

  • In total 177 individuals from 9 localities of Belgorod region were studied by molecular genetic method: 1. «Severskiy Donec» 50.591/36.608 (n=14); 2

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Summary

Introduction

The European green frogs complex (Pelophylax esculentus complex) includes three species: Pelophylax ridibundus (Pallas, 1771), P. lessonae (Camerano, 1882) and P. esculentus (Linnaeus, 1758) [1]. According to studies by a number of authors, hybrid frogs are distributed almost all over Europe; this fact may indicate their "evolutionary success". Hybrids and their parents often live together and form common breeding groups, despite differences in their lifestyle [2]. Accurate identification of species of the European green frogs complex is difficult, and hybrid species often erase the "boundaries" of parent species, but it is this necessary to solve a number of problems: ecological monitoring, study of species introduction and invasion and to develop a strategy for their conservation [3, 4]. The aim of the study is a reliable species identification of the European green frogs Pelophylax esculentus complex in the Belgorod and its surroundings

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