Species and concentrations of selenium and nutrients in Tanabe Bay during red tide due toGymnodinium nagasakiense

Abstract

A red tide due toGymnodinium nagasakiense was observed in August 1988 in Tanabe Bay, Wakayama Prefecture, Japan. The maximum cell concentration ofG. nagasakiense reached 1×105 cells ml−1 at the surface water. From May to September 1988, the following were monitored: water temperature, salinity, chlorophylla, D.O., dissolved nutrients (NO2−N, NO3−N, NH4−N, PO4−P DON, DOP), particulate nutrients (PON, POP) and three dissolved selenium species [Se(IV), Se(VI), Organic Se]. Dissolved inorganic nitrogen (NO3−N, NH4−N) decreased but PON, POP, DON, DOP and inorganic phosphate increased at the peak of the bloom. The concentration of organic selenium increased up to the bloom initiation period which started on 5 July, and then the concentration of Se(IV) increased as the concentration of organic selenium decreased at the peak of the bloom (3 August). The strong relationship was found between the concentration of Se(IV) and the cell concentration ofG. nagasakiense (r2=0.98). The Se(IV) requirement ofG. nagasakiense was 2.89×10−17 moles cell−1, which was agreed well with 4.4×10−17 moles cell−1 found in a laboratory experiment onG. nagasakiense using selenium spiked artificial sea water medium. The average ratio of Se(IV) to dissolved inorganic nitrogen (DIN) during the red tide bloom was 1∶1441, the ratio of Se(IV) to DIN at the surface with the maximum cell concentration ofG. nagasakiense of 1×105 cells ml−1 was 1∶137. These results suggested that selenium may play an important role in red tide outbreak ofG. nagasakiense.