Speciation of Thulium Porphyrin Complexes by Reversed‐Phase HPLC

Abstract

Six new thulium porphyrin complexes have been separated by high performance liquid chromatography with triethanolamine (TEOLA) and acetic acid (HAc) as additives in the methanol–water mixture. The function of TEOLA and HAc has been described. The whole analysis was completed on a C18 column in 16 min at a flow rate of 0.5 mL min−1, with the mobile phase of methanol–water (93:7,v:v) containing 75 mmol L−1 TEOLA and 200 mmol L−1 HAc. The detection limits (S/N = 3) of thulium porphyrins at 420 nm were 1.0, 1.0, 1.0, 1.0, 5.0, and 5.0 ng mL−1, respectively. Compared with the methanol system, it can attain the equivalent separation with the mobile phase of ethanol–water (90:10,v:v) containing 25 mmol L−1 TEOLA and 200 mmol L−1 HAc pumped at 0.25 mL min−1.