Abstract
A simple in-vitro speciation of inorganic selenium (SeIV and SeVI) in serum blood and urine of hyperthyroidism and hypothyroidism patients based on isopropyl 2-[(isopropoxycarbothiolyl) disulfanyl] ethane thioate (IICDET) as a complexing agent were studied by ultrasound-assisted dispersive liquid-liquid bio-microextraction procedure (USA-DLLMBE). In first stage, 100 μL (≈0.1 g) of hydrophobic ionic liquid of [C8MIM][PF6] mixed with IICDET ligand and 100 μL of acetone. Then, the mixture injected to 10 mL of samples at pH=4. After shacking, the Se (IV) ions were complexed by IICDET and extracted to IL at pH=4 (R-S: …Se). The IL phase was separated from sample by centrifuging and inorganic selenium (Se IV) in remained samples was determined by electro thermal atomic absorption spectrometry (ET-AAS) after back extraction of Se (IV). As speciation, the Se (VI) reduced to Se (IV) in acidic pH (HCl, 130OC) and the total Se(T-Se) was obtained at pH=4.
Highlights
Selenium is an essential trace element in humans
One of the diseases that affect the thyroid gland is subclinical hypothyroidism, which is characterized by elevated serum levels of thyroid-stimulating hormone (TSH) at a concentration recommended for prohormone thyroxine (T4) and active hormone triiodothyronine (T3)
The human blood, serum and urine samples based on isopropyl 2-[(isopropoxycarbothiolyl) disulfanyl] ethane thioate (IICDET) was used for selenium speciation with high accuracy by USA-DLLMBE procedure
Summary
Selenium is an essential trace element in humans. The soluble selenium compounds can be absorbed through the lungs and the gastrointestinal tract. A risk of diabetes has been reported following long-term selenium supplementation, but few data are available on the side effects associated with such supplementation and further studies are required. Selenium species in serum and urine must be evaluated and determined by favorite techniques. The different methods such as, flame atomic absorption spectrometry [18], electrothermal atomic absorption spectrometry [19], liquid chromatography and liquid chromatography inductively coupled plasma mass spectrometry (LC and LC-ICP- MS) [20,21,22] and high-performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry [23,24] were used for Se determination in different human and water samples. Validation methodology was confirmed by spiking of standard samples and ICP-MS
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