Speciation of chromium in blood samples based on dithioglycerol immobilized on carbon nanotube by dispersive micro solid phase bioextraction

Eskandar Kolvari,Jamshid Rakhtshah,Nafiseh Esmaeili

doi:10.24200/amecj.v3.i03.114

Eskandar Kolvari, Jamshid Rakhtshah + Show 1 more

Open Access

https://doi.org/10.24200/amecj.v3.i03.114

Copy DOI

Abstract

A novel method based on the synthesis of dithioglycerol immobilized on carbon nanotubes (CNTs@DTG) was used for speciation of chromium (Cr III and Cr VI) in human blood samples by dispersive micro solid-phase bio extraction (D-μ-SPBE). By procedure, a mixture containing acetone, 1-octyl-3-methylimidazolium hexafluorophosphate ([OMIM][PF6]), and CNTs@DTG were injected into 5 mL of the standard sample containing 1.0 μg L-1 of Cr III and Cr VI which was diluted with DW up to 10 mL at optimized pH. The Cr (VI) anions and Cr (III) cations were efficiently extracted by HS of CNTs@DTG at pH 2 and 6, respectively (HS.....Cr), and trapped into IL phase at the bottom of the conical tube. Then, Cr (III) and Cr(VI) ions were back-extracted before determined by electrothermal atomic absorption spectrometry (ETAAS). The enrichment factor (EF), linear range, and limit of detection (LOD) were obtained 9.85, 0.12-3.88 μg L-1, and 30 ng L-1, respectively.

Highlights

Heavy metals accumulate in different human tissues as non biodegradability property
The ionic liquids (ILs) as an organic salt has various advantages such as low vapor pressure, thermal stability about 200-350 °C, large viscosity, good extractability and separation phase [21]. The different adsorbents such as multi-walled carbon nanotubes (MWCNTs) [22], silica nanoparticles [23], MIL-101(Fe) and dithiocarbamate-modified magnetite nanoparticles [24], and fabrication of magnetic particles imprinted cellulose based biocomposites [25] were used for extraction and separation of chromium ions in a different matrix
Experimental parameters were optimized for chromium speciation, and the performance of the proposed method was evaluated by ET-AAS

Summary

Introduction

Heavy metals accumulate in different human tissues as non biodegradability property. Many of them enter to the human body from foods and waters and absorb them by the gastrointestinal system. As different toxicity and exposure, the favorite and efficient procedure must be used for chromium speciation. The ILs as an organic salt has various advantages such as low vapor pressure, thermal stability about 200-350 °C, large viscosity, good extractability and separation phase [21] The different adsorbents such as multi-walled carbon nanotubes (MWCNTs) [22], silica nanoparticles [23], MIL-101(Fe) and dithiocarbamate-modified magnetite nanoparticles [24], and fabrication of magnetic particles imprinted cellulose based biocomposites [25] were used for extraction and separation of chromium ions in a different matrix. The ICP-MS (Perkin Elmer, USA) as ultra-trace analysis with high sensitivity was used for determining of chromium ions in human blood samples (1200 W; 1.0 L min-1; 2.0 sec per mass; auxiliary gas 1.2 L min). An ultrasonic bath for molecular biology such as blood samples with temperature controlling in realtime was prepared (Thomas, HB120 LED digital dry bath, USA)

Wavelength Slit Lamp current Injection mode Volume Injection Mode

Si O MeO O

Results and discussion

Conclusion