Speciation in Sapsuckers (Sphyrapicus): I. Genetic Differentiation

Abstract

Abstract We report the results of an electrophoretic analysis at 39 presumptive genetic loci of 88 specimens in the picid genus Sphyrapicus, here treated as comprising the following species: Williamson's Sapsucker (S. thyroideus), Yellow-bellied Sapsucker (S. varius), Red-naped Sapsucker (S. nuchalis), and Red-breasted Sapsucker (S. ruber). Seventeen loci (43.5%) were polymorphic. Values for observed heterozygosity in S. ruber ruber, S. ruber daggetti, and in three populations of S. nuchalis were uniform and averaged 0.043, the mean value reported for other birds. However, S. varius, at 0.022, and S. thyroideus, at 0.016, have low values of H. Values for percentage of polymorphic loci ranged from 12.8 to 20.5 in S. ruber and S. nuchalis, 12.8 in S. varius, and 7.7 in S. thyroideus. In contrast, all four species exhibited a similar mean number of alleles per locus (1.12-1.21). Genetic distances between populations of the same species are either very low or zero. Genetic distances are also very low between the phenotypically dissimilar ruber and nuchalis (D̄ = 0.004), two forms we consider to be biological species based on their assortative mating in sympatry. This is the lowest avian interspecific D-value ever reported and is similar to that found between avian subspecies. Phenotypically, varius and nuchalis are very similar, yet the average genetic distance between samples of these forms, 0.029, is comparable to interspecific values reported for other avian congeners and is our chief basis for treating the two forms as separate species. S. thyroideus is strongly differentiated genetically from varius (D̄ = 0.142), from nuchalis (D̄ = 0.197), and from ruber (D̄ = 0.186). FST statistics point to little or no population subdivision within ruber and nuchalis but substantial subdivision within the superspecies varius (S. varius + S. nuchalis + S. ruber). The genetic information suggests that the Williamson's Sapsucker represents the oldest lineage in the genus. S. thyroideus split from its sister clade (superspecies S. varius) between 3 and 4 MYBP. S. ruber and S. nuchalis are sister species that evolved very recently, either during or since the Pleistocene and sometime after their common ancestor diverged from S. varius.