Abstract
A miniaturized fluorescent spectrophotometer was constructed in this work with a cheap light-emitting diode (LED) as the light source and a handheld charge coupled device (CCD) as the detector. The developed system was utilized to accurately detecting silver ions (Ag+) and silver nanoparticles (AgNPs) without any pre-concentration and chromatographic separation operations. The principle of this speciation analysis was that Ag+ can quench the fluorescent of CdTe QDs, while AgNPs cannot. Under the optimal conditions, the limit of detection (LOD) was found to be 0.06 μg mL−1 for both Ag+ and AgNPs, and the relative standard deviation (RSD, n = 7) was calculated to be 2.9% for 1 μg mL−1 of Ag+. The proposed system was demonstrated for the speciation analysis in commercial antibacterial products with satisfactory results.
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