SPE–LC Multi-Residue Analysis of Mebendazole and its Metabolites in Grass Carp and Shrimp

Abstract

An analytical method was developed to detect the residue of mebendazole and its metabolites (hydroxymebendazole and aminomebendazole) in the muscle of grass carp and shrimp by LC–UV detection. Mebendazole and its metabolites were extracted with water and ethyl acetate, defatted with hexane, and purified with MCX solid phase extraction column. The intra- and inter-batch precision (measured by CV%) was <9.0%. The accuracy (measured by relative error, %) was <12%. The LODs were 2.5 μg kg−1 for mebendazole and hydroxymebendazole, 5 μg kg−1 for aminomebendazole; the LOQs were 5 μg kg−1 for mebendazole and hydroxymebendazole, 10 μg kg−1 for aminomebendazole. The mean recoveries of mebendazole and its metabolites from grass carp and shrimp muscle at a concentration range of 5.0–500.0 μg kg−1 were 90.7–97.0% with relative standard deviations below 10%.