Abstract
Attempts were made to spawn Clarias batrachus by injecting human chorionic gonadotropin (HCG) at different doses (1000, 2000, 3000, 4000 and 5000 IU/kg body weight) in combination with latency periods (11,14, 17, 20 and 23 h). The females could not be stripped at 11 h latency when injected with 1000–3000 IU of HCG per kg body weight. A significant ( P < 0.05) reduction of stripped egg number was observed when females were injected with a dose of 5000 IU HCG and stripped at 20–23 h post-injection. The eggs obtained from the females with injection of 4000–5000 IU per kg body weight and stripped at 11 h post-injection, did not fertilize. The highest fertilization (75–89%) and hatching (66–78%) could be obtained at 3000–5000 IU doses of HCG with 14–17 h post-injection. Although the highest working fecundity was obtained with 4000 IU HCG at 14 and 17 h latency, the response from 3000 IU HCG was only slightly less at these latency periods, and was effective for up to 23 h.
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