Spatiotemporal variation in thermal niches suggests lability rather than conservatism of thermal physiology along an environmental gradient

Abstract

Abstract Temperature variation throughout a species range can be extensive and exert divergent spatiotemporal patterns of selection. The estimation of phenotypic differences of populations along environmental gradients provides information regarding population-level responses to changing environments and evolutionary lability in climate-relevant traits. However, few studies have found physiological differentiation across environmental gradients attributable to behavioural thermoregulation buffering physiological evolution. Here, we compared thermal sensitivity of physiological performance among three populations of the ornate tree lizard (Urosaurus ornatus) along a 1100 m elevational gradient in southeastern Arizona across years in order to determine whether spatial differences in thermal environments are capable of driving local physiological differentiation. Lizards exhibited significant population-level differences in thermal physiology. The thermal traits of lizards at low elevations included warmer body temperatures and higher preferred and critical thermal temperatures. In contrast, lizards at higher elevations had cooler body temperatures and lower preferred and critical thermal temperatures. Populations also exhibited differences in the optimal temperature for performance and thermal performance breadth. The direction of population variation was consistent across years. Environmental gradients can provide model systems for studying the evolution of thermal physiology, and our study is one of the first to suggest that population differentiation in thermal physiology could be more prominent than previously thought.