Spatiotemporal properties of whisker-evoked tactile responses in the mouse secondary somatosensory cortex

Sophie Hubatz,Isabelle Férézou,Guillaume Hucher,Daniel E Shulz

doi:10.1038/s41598-020-57684-6

Abstract

The representation of rodents’ mystacial vibrissae within the primary somatosensory (S1) cortex has become a major model for studying the cortical processing of tactile sensory information. However, upon vibrissal stimulation, tactile information first reaches S1 but also, almost simultaneously, the secondary somatosensory cortex (S2). To further understand the role of S2 in the processing of whisker inputs, it is essential to characterize the spatio-temporal properties of whisker-evoked response dynamics in this area. Here we describe the topography of the whiskers representation in the mouse S2 with voltage sensitive dye imaging. Analysis of the spatial properties of the early S2 responses induced by stimulating individually 22 to 24 whiskers revealed that they are spatially ordered in a mirror symmetric map with respect to S1 responses. Evoked signals in S2 and S1 are of similar amplitude and closely correlated at the single trial level. They confirm a short delay (~3 ms) between S1 and S2 early activation. In both S1 and S2 caudo-dorsal whiskers induce stronger responses than rostro-ventral ones. Finally, analysis of early C2-evoked responses indicates a faster activation of neighboring whisker representations in S2 relative to S1, probably due to the reduced size of the whisker map in S2.

Highlights

The representation of rodents’ mystacial vibrissae within the primary somatosensory (S1) cortex has become a major model for studying the cortical processing of tactile sensory information
Since the first description of its remarkable cellular organization by Woolsey and Van der Loos[1], the rodent primary somatosensory (S1) cortex has become a major model for studying the cortical treatment of tactile sensory information[2,3,4]
In order to provide a thorough description of the topography of the whiskers representation in the mouse S2, and a quantitative comparison of the dynamics of single whisker-evoked responses between S1 and S2, we used

Summary

Introduction

The representation of rodents’ mystacial vibrissae within the primary somatosensory (S1) cortex has become a major model for studying the cortical processing of tactile sensory information. Quantification of the fluorescent signals from ROIs defined by using the above-described mapping method was used to compare responses to individual whisker stimulations in S2 versus S1 (Fig. 3).

Results

Conclusion