Spatiotemporal dynamics of fibrin formation and spreading of active thrombin entering non-recalcified plasma by diffusion

Abstract

The spatiotemporal dynamics of clot growth was studied in non-stirred non-recalcified plasma where thrombin entered by diffusion. Under these conditions, the clot rapidly grew for 30–45 min and then stopped growing on reaching 0.4–0.5 mm in size. The dynamics of clot growth and its size almost did not depend on the thrombin concentration in the range from 50 to 400 nM. FITC-thrombin was shown to permeate the growing clot. The clot size in antithrombin-deficient plasma increases with decreasing antithrombin concentration, being 1.5 mm in the plasma depleted of antithrombin to 5% of its initial level. The data on the spatial distribution of amidolytic activity in the growth zone of the clot suggested that thrombin was not the sole source of this activity. Analysis showed that this additional activity arising during thrombin diffusion into plasma was largely accounted for by thrombin-α 2-macroglobulin complex.