Spatio-Temporal Variations in Pathogenic Bacteria in the Surface Sediments of the Zuari Estuary, Goa, India

Abstract

Estuaries are hotspots of anthropogenic activities. The deposition of pathogenic bacteria in the sediment and their re-suspension into the water column are influenced by riverine discharge and tides. The abundance of Escherichia coli O157:H7, Shigella spp., Salmonella spp., total coliforms (TC) and Vibrio spp. ( Vibrio cholerae (VC), Vibrio parahaemolyticus (VP), Vibrio alginolyticus (VA)) was assessed along with the total bacterial count (TBC) and total viable count (TVC) in surface sediments along the banks of the Zuari estuary, Goa, India. The study was carried out fortnightly for a period of 17 months covering three seasons, i.e. pre-monsoon (PreM), monsoon (MON) and postmonsoon (POM). The spatial and temporal changes in the quality of organic matter were also assessed. The organic matter content was high and rich in carbohydrates and proteins towards upstream sites. The quality of organic matter was influenced by the seasons. E. coli O157:H7 was detected only during MON towards the upstream stations. A negative correlation between TC and TBC with salinity was evident indicating the influence of land run-off. The Shigella spp. and VA were high towards the mouth of the estuary during PreM. However, during POM, the TVC, TC and VP were abundant towards the upstream and VC were abundant at the mouth of the estuary. Among the Vibrios, VP and VA were the most frequently occurring bacteria whereas TC and Shigella spp. were dominant among allochthonous pathogens in the sediments irrespective of space and time. In addition to influence of seasons, the sampling time influenced by tidal condition also played an important role in the population dynamics of pathogenic bacteria in the sediments. Future studies should address the interaction of pathogenic bacteria with suspended particles, their transport and survival in the sediments.