Spatial variability and evaluation of airborne bacteria concentration in manure belt poultry houses

Abstract

Manure belt poultry houses were widely used for egg production. However, reliable spatial distribution of airborne bacteria data from long-term on-farm monitoring in manure belt houses are scarce. In this study, the airborne bacteria were collected by the Andersen air microorganism sampler in a 4 tiers and an 8 tiers layer house, respectively. Results revealed that the airborne bacteria concentration range from 565 ± 247 CFU/m3 to 12,118 ± 883 CFU/m3 inside the 4 tiers poultry farmhouse and 459 ± 247 to 12,966 ± 884 CFU/m3 inside 8 tiers poultry farmhouse, respectively. The average airborne bacterial concentrations in the 4 and 8 tiers manure belt houses were 4,527 ± 2,509 and 5,489 ± 2,579 CFU/m3, respectively. Significant spatial variations of airborne bacteria concentration were observed for both houses. Spatial distribution of airborne bacteria concentration along the long axis direction can be divided into 3 regions: the low concentration region (<6,000 CFU/m3), the transition region (6,000-10,000 CFU/m3), and the high concentration region (>10,000 CFU/m3), and airborne bacteria concentration was symmetrically distributed along the short axis direction inside both houses. We used 5 and 3 sampling locations to assess the average and maximum airborne bacterial concentration inside the manure belt houses, respectively. The average airborne bacteria concentration of 3 sampling locations was closest to the maximum concentration of both houses. It is more useful to use 3 sampling locations to monitor the change of the maximum airborne bacterial concentration inside the manure belt houses.