Spatial turnover of core and occasional bacterial taxa in the plastisphere from a plateau river, China

Abstract

Plastic surfaces in the environment are a comparatively new niche for microbial colonization, also known as the “plastisphere”. However, our understanding of the core and occasional bacterial taxa in the plastisphere is limited. Here, environmental plastic, water, and sediment samples were collected from 10 sites in a plateau river (Lhasa River, China) in September of 2019. The composition and spatial turnover of core and occasional bacterial taxa in the plastisphere were revealed via 16S rRNA gene sequencing and compared with water and sediment. The results indicated that deterministic processes dominated the habitat specialization that shaped the formation of core and occasional taxa in the plastisphere, water, and sediment of the Lhasa River because the decline in zeta diversity in the plastisphere, water, and sediment was more fitted to a power-law form rather than an exponential form. Proteobacteria (65.9%), Bacteroidetes (16.0%), and Cyanobacteria (11.7%) dominated the plastic core taxa. Core taxa rather than occasional taxa in the plastisphere had a lower (21.7%) proportion of OTUs and a higher (81.7%) proportion of average relative abundance than water and sediment, which were dominant in plastic bacterial communities. The spatial turnover of core and occasional bacterial taxa in the plastisphere was governed by abiotic as well as biotic factors. Specifically, the spatial turnover of core taxa in the plastisphere with high connectivity but low functional redundancy was easily affected by geographical distance, altitude, and heavy metals. Furthermore, strong drug resistance was found in the spatially persistent core taxa in the plastisphere. This study provides empirical support for the spatial turnover (species variation) and potential ecological mechanisms of bacterial communities in the plastisphere from river ecosystems.