Abstract

By depositing a resolution test pattern on top of a Si3N4 photonic crystal resonant surface, we have measured the dependence of spatial resolution on refractive index contrast Δn. Our experimental results and finite-difference time-domain (FDTD) simulations at different refractive index contrasts show that the spatial resolution of our device reduces with reduced contrast, which is an important consideration in biosensing, where the contrast may be of order 10−2. We also compare 1-D and 2-D gratings, taking into account different incidence polarizations, leading to a better understanding of the excitation and propagation of the resonant modes in these structures, as well as how this contributes to the spatial resolution. At Δn = 0.077, we observe resolutions of 2 and 6 μm parallel to and perpendicular to the grooves of a 1-D grating, respectively, and show that for polarized illumination of a 2-D grating, resolution remains asymmetrical. Illumination of a 2-D grating at 45° results in symmetric resolution. At very low index contrast, the resolution worsens dramatically, particularly for Δn < 0.01, where we observe a resolution exceeding 10 μm for our device. In addition, we measure a reduction in the resonance linewidth as the index contrast becomes lower, corresponding to a longer resonant mode propagation length in the structure and contributing to the change in spatial resolution.

Highlights

  • Photonic crystal biosensors based on resonant surfaces have been developed over the past decade for highly sensitive label-free sensing [1]–[7]

  • We have studied the spatial resolution of a silicon nitride resonant surface grating biosensor by depositing a resist pattern on to the surface

  • We study the response of a 2-D grating being illuminated with different orientations of polarization

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Summary

Introduction

Photonic crystal biosensors based on resonant surfaces have been developed over the past decade for highly sensitive label-free sensing [1]–[7]. Of view, enabling the imaging of surface binding events by combining spatial information with sensing information This combination has added a powerful new imaging modality to the biophotonics toolkit; for example, it allows high-throughput DNA-binding and gene-expression assays [1], it enables determination of cellular attachment to a surface [2], and indication of cell behaviour with respect to a given treatment [4]; it has been shown to enhance fluorescence imaging efficiency [6], [10]; and it could enable the imaging of cellular secretion of specific molecules without the use of fluorescent labels. The dependence of spatial resolution on refractive index contrast has not been studied yet, and the impact of polarization has not been quantified. When imaging, for example, living cells in culture media, the refractive index contrast is typically small: on the order 10À2 [14]

Experimental Details
Spatial Resolution Measurements
Dependence on Refractive Index Contrast
Findings
Conclusion and Discussion
Full Text
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