Abstract
Analysis of the transcriptional activity in Escherichia coli K12 revealed an asymmetry in the distribution of transcriptional patterns along the bacterial chromosome and showed that spatial patterns of transcription could be modulated pharmacologically and genetically.
Highlights
Genes on the chromosome are organized in a fixed order, the spatial correlations in transcription have not been systematically evaluated
Available structural information about bacterial chromosomes indicates that the chromosome is supercoiled in vivo [2] and organized in topologically constrained domains [3]; diffusion of supercoils over the chromosome is impeded in actively replicating cells [4]; the chromosome is mildly condensed in vivo [5,6]; chromosomal loci inside the cell are organized and arrayed in linear order according to the linear genetic map [7,8]; and at least two chromosomal loci are actively moved and positioned inside the cell [7,9]
We examined the distribution of DNA gyrase along the chromosome in a chromatin immunoprecipitation (ChIP) chip assay with GyrA-specific antibodies
Summary
Genes on the chromosome are organized in a fixed order, the spatial correlations in transcription have not been systematically evaluated. Available structural information about bacterial chromosomes indicates that the chromosome is supercoiled in vivo [2] and organized in topologically constrained domains [3]; diffusion of supercoils over the chromosome is impeded in actively replicating cells [4]; the chromosome is mildly condensed in vivo [5,6]; chromosomal loci inside the cell are organized and arrayed in linear order according to the linear genetic map [7,8]; and at least two chromosomal loci are actively moved and positioned inside the cell [7,9]. Global transcriptional profiles have been successfully used to probe the organization of transcriptional units into operons [11] and regulons [12,13]. Such analysis is limited by assumptions about the nature of transcriptional units
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