Spatial organization of digestion in the larval and imaginal stages of the sciarid fly Trichosia pubescens

Abstract

Aminopeptidase, amylase, cellulase and trypsin are found in major amounts in the midgut lumen, whereas alkaline phosphatase, cellobiase, α-glucosidase, maltase and trehalase occur mainly in the midgut tissue of Trichosia pubescens larvae. Cellulase and a part of the amylase seem to be derived from the fungi the larvae eat. Based on the molecular weights of the enzymes which pass and of those which do not pass through the peritrophic membrane, it is possible to estimate the peritrophic membrane pores as having diameters of 7.5–8.0 nm. Purification and assays of microvillar enzymes from different larval midgut regions suggest that alkaline phosphatase, cellobiase, α-glucosidase, maltase and trehalase are bound to the plasma membrane chiefly of midgut caeca cells. The results support the hypothesis that digestion starts in the endoperitrophic space under the action of amylase, cellulase and trypsin, goes on in the ectoperitrophic space by amylase, cellulase and aminopeptidase and is completed through the catalytic action of plasma membrane-bound hydrolases. The data lead to the conclusion that the spatial organization in T. pubescens larvae is identical to that of another Sciarid fly ( Rhynchosciara americana) despite the finding that the midgut trehalase is bound to the plasma membrane in T. pubescens and soluble in R. americana. With metamorphosis salivary amylase appears, α-glucosidase, trehalase and maltase increase, and the other midgut hydrolases decrease or even disappear. This is in accordance with the fact that the larvae feed on decaying plants and fungi and the imagoes feed on nectar.