Spatial learning and memory impairment in an acute experimental autoimmune encephalomyelitis

Abstract

Event Abstract Back to Event Spatial learning and memory impairment in an acute experimental autoimmune encephalomyelitis Dejana Kosanovic1*, Aleksandra Stojkovic1, Natasa Vukov1 and Katica Jovanova-Nesic1 1 Biomedical Center of the Institute Torlak, Neuroimmunology, Serbia Experimental autoimmune encephalomyelitis (EAE) could be associated with cognitive disturbances, so the aim of the study was investigation of spatial learning in mice with EAE. EAE was induced in eight-week-old C57BL/6 female mice by subcutaneous immunization (MOG35-55 in complete Freund’s adjuvant) and pertussis vaccine intraperitoneally. Animals were scored daily for clinical signs of EAE. Morris water maze test (MWM), consisting of a circular tank filled with water and a submerged platform in a fixed spot was performed before and after EAE induction. The path, time, and motor function during MWM test were observed. Hematoxylin-eosin staining of brain tissue was used for histological examination. After two trials mice mostly swam in the quadrant with the platform and after 8 training days all the mice swam directly to the platform. 50 % of mice with EAE showed partial loss of tail tonicity approximately 13 days post immunization. In all mice with EAE hind limb weakness after swimming was observed 18 days post immunization. Significant difference (p<0.05) in the time for finding the platform was found between mice with mild clinical signs of EAE in comparison with other mice (EAE without symptoms and non-treated). Interindividual behavioral difference within mice with EAE signs were observed, in 40 % there were no changes while in 60 % signs of disorientation and anxiety-like behavior was noted. Histopathological examination showed lesions in the brain parenchyma, especially in the hippocampal region of EAE mice. Hippocampal tissue destruction in EAE mice indicates the cognitive learning and memory impairment detected. Keywords: EAE, Morris water maze, Spatial learning, memory loss, Animal Models Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Kosanovic D, Stojkovic A, Vukov N and Jovanova-Nesic K (2013). Spatial learning and memory impairment in an acute experimental autoimmune encephalomyelitis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00169 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Dejana Kosanovic, Biomedical Center of the Institute Torlak, Neuroimmunology, Belgrade, 11000, Serbia, dr.dejana@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Dejana Kosanovic Aleksandra Stojkovic Natasa Vukov Katica Jovanova-Nesic Google Dejana Kosanovic Aleksandra Stojkovic Natasa Vukov Katica Jovanova-Nesic Google Scholar Dejana Kosanovic Aleksandra Stojkovic Natasa Vukov Katica Jovanova-Nesic PubMed Dejana Kosanovic Aleksandra Stojkovic Natasa Vukov Katica Jovanova-Nesic Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.