Spatial dynamics of yeast community and its relationship to environmental factors in Lake Fuxian, Yunnan Province

Abstract

开展高原湖泊酵母菌多样性研究,能够为湖泊生态系统的保护提供理论依据,并为其中特殊酵母菌资源的开发及利用奠定基础.结合经典分类法及26S rDNA D1/D2区域序列分析,对分离自云南抚仙湖湖水中的553株酵母菌进行系统分类,运用SPSS 19.0软件比较不同区域酵母菌多样性,并采用多元统计方法定量分析酵母菌空间分布特征及其与理化因子之间的关系.结果显示:抚仙湖水体中分布22属52种和1个潜在新分类单元的酵母菌.理化因子差异性分析表明,北沿岸区总有机碳浓度明显高于南沿岸区.Pearson分析则表明,抚仙湖湖水总有机碳浓度与酵母菌丰度呈显著正相关.另外,酵母菌-环境冗余分析显示,抚仙湖酵母菌种群结构与总有机碳浓度存在明显相关.研究显示云南抚仙湖酵母菌资源比较丰富,人类活动对其中酵母菌空间分布具有一定影响.;Studying on yeast diversity in plateau lakes can provide theoretical basis for protection of lake ecosystem and lay foundation for exploitation of yeast resouses.A total of 553 yeast isolates were obtained from Lake Fuxian in Yunnan Province. Compared yeast diversity indices in different regions by SPSS statistics 19.0, and spatial dynamics of yeast community and its relationship to environmental factors were evaluated.The results showed that yeasts were identified as belonging to 22 genera and 52 species and 1 presumed new species by 26S rDNA D1/D2 domain sequence analysis.Duncan test showed that Pielou evenness index of yeasts existed in southern region was higher than that of Northern region distinctly.Difference analysis revealed that content of total organic carbon in Northern district was more than that in Southern district, besides, Pearson correlation analysis displayed that content of total organic carbon was significantly positive related to yeast abundance. Redundancy analysis revealed that yeast community in Lake Fuxian was obviously in relation to content of total organic carbon. The study showed that yeast resourses in Lake Fuxian were abundant,in addition,human activities had influence on spatial dynamics of yeast community there.