Spatial distribution of smooth muscle tissue in the female pelvic floor and surrounding the urethra and vagina.

Abstract

Data regarding urethral supporting structures are insufficient for understanding the mechanism of stress urinary incontinence. Whether smooth muscle fibers contribute to urethral support and pelvic floor support structures is unclear. This study aimed to clarify the histological structures and spatial distributions of smooth muscle tissues surrounding the urethra and vagina. Using cadaveric specimens, macroscopic anatomical and histological evaluations were conducted. Six female cadavers were used for macroscopic observations. Ten female cadavers were used for histological observations. Three pelvises were cut in a plane vertical to the urethra, and the other pelvises were cut in a plane parallel to the urethra and vagina to observe tissues surrounding the urethra and vagina. The major tissue component around the proper muscle layer of the urethra was smooth muscle tissue, which mediated among the urethra, pubis, and levator ani. Smooth muscle tissues laterally extended the smooth muscle fibers, both superiorly and inferiorly toward the levator ani, with a few fibers inserted in the levator ani. Smooth muscle was found between the urethral walls and pubic bones. Smooth muscle may contribute to the mechanism of pelvic floor support.