Abstract
Immunohistochemical stains of soybean cotyledon sections indicated higher levels for both the glycinins and the β-conglycinins in the abaxial as compared to the adaxial region of the cotyledon, but more equal distribution for the Kunitz and the Bowman-Birk trypsin inhibitors. This spatial pattern was verified by electrophoretic and immunochemical analysis of extracts made from dissected cotyledons. Examination of seeds at various stages of seed development showed that the spatial pattern was already established when the seeds had accumulated half of the storage proteins. Although absolute amounts were different, the relative protein composition found in both the abaxial and adaxial regions of the cotyledons of the developing seed did not differ to a great extent.
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