Abstract
The aim of this study was to evaluate effects of intra-follicular (i.f.) treatment with lipopolysaccharide (LPS) on follicular and luteal development in cows. There were 18 non-lactating cows assigned to two groups to address this aim: control group (n = 9), which received an i.f. injection of saline; and LPS group (n = 9), which received an i.f. injection of 1 μg of LPS per mL of follicular fluid. Cows were treated with an intravaginal P4 releasing device (IVD) and estradiol benzoate on D0. On D4 and D5 cows were treated with cloprostenol sodium and on D7 the IVD was removed. At 12 h after IVD removal, cows were administered the i.f. injection of LPS or saline. After administration of these treatments, follicular development was evaluated every 12 h until ovulation. The LPS treatment increased blood flow in pre-ovulatory follicles (P = 0.05). Follicle growth was reduced by LPS injection (P < 0.02) resulting a longer period to the time of ovulation for cows in the LPS than control group (P = 0.03). The percentage of cows having ovulations was less for the LPS than control group (P = 0.03). The diameter of the CL, CL blood flow and P4 concentrations 5 and 12 days after ovulation did not differ between groups (P> 0.05). In conclusion, intra-follicular treatment with LPS resulted in a decreased rate of follicle growth, delayed timing of ovulations and a lesser number of cows having ovulations.
Highlights
During the reproductive lifespan, a rich and finite number of gametes are available and represent the main oocyte ovarian reserve of an individual
Regardless of ovarian portion, a greater proportion (~92%) of preantral follicles was found in the circle of 10 mm radius from the geometric center
Within the same distance (
Summary
A rich and finite number of gametes (primordial follicles) are available and represent the main oocyte ovarian reserve of an individual. Folliculogenesis in vivo is a complex dynamic process featured by follicular quiescence [1], activation and growth [2], follicular migration [3], and cell interactions [4]. Once these events occur simultaneously in the ovarian parenchyma [5], studies related to preantral follicle spatial distribution can potentially help to clarify important anatomical-physiological mechanisms involved in folliculogenesis.
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