Spatial distribution and viability of Alexandrium tamarense resting cysts in surface sediments from the St. Lawrence Estuary, Eastern Canada

Abstract

The dinoflagellate Alexandrium tamarense Group 1 (as defined by Lilly et al., 2007) is responsible for recurrent outbreaks of paralytic shellfish poisoning (PSP) in the St. Lawrence Estuary (SLE), Eastern Canada. In August 2008, a major bloom of A. tamarense developed in the SLE and caused major mortality of fish, seabirds and marine mammals notably in the vicinity of a marine park. Eleven months later, surface (0–5 cm) and deeper (5–10 cm) sediments were sampled to determine resting cysts concentrations, locate prospective cyst seedbeds and examine if these had changed following this major bloom. This information is thought to be important to understand inter-annual patterns in algal toxicity, cyst abundance being a good predictor of subsequent bloom magnitude in some regions. Surface cyst distribution was heterogeneous and it confirmed the location of the cyst seedbed previously reported on the north shore near the Manicouagan/aux-Outardes Rivers (>500 cysts cm−3). A zone of cyst accumulation was also observed on the south shore of the SLE (maximum of 1200 cysts cm−3), with higher concentrations relative to previous cyst mapping in the 1980s. A mismatch was observed between the zones with high surface cyst concentrations and those where the highest PSP toxins were detected (used as a proxy for vegetative cells in the water column). Cyst concentrations were negatively correlated with PSP levels from the same sites, suggesting that cysts were formed and deposited away from the major sites of toxicity. Deposition likely took place near the end of the bloom, once it had reached the eastern boundary of the SLE. PSP toxicity was worse near the peak of the bloom, which occurred westward of this region. This highlights the dynamic behaviour of local blooms, influenced by the estuarine and mesoscale circulation. Interestingly, the major bloom of August 2008 was not followed by particularly large cyst deposition or by any major bloom in 2009 in this region. Cyst viability was also examined, using Sytox Green, and found to be highest (nearly 100%) in the sites where cyst concentrations were maximum. Our results call for further investigation of the cyst mapping hypothesis in the St. Lawrence, where the local circulation seems to have an overriding influence on cyst deposition patterns.