Abstract
The stability of glucose oxidase (GOx) immobilized on a silicon electrode can be enhanced using spatial confinement as described in this paper. We show that when GOx is assembled in semiopen spatially confined structures created on the unmodified surface of a silicon electrode, the enzyme's activity is preserved under destabilizing conditions. GOx-immobilized silicon electrodes were treated with guanidinium chloride and high temperature. Cyclic voltammetry measurement of the treated electrodes showed the proper redox characteristics of GOx and indicated the GOx-catalyzed electrooxidation of glucose. When GOx was immobilized on flat silicon electrodes, voltammetric measurement showed null result presumably caused by denaturation of the enzyme. The effect of spatial confinement on enzyme stability is also revealed by analyzing the characteristic rate constants and the kinetic parameter for the complete catalytic process of glucose.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
