Abstract
Spatial and temporal variability in antibiotic resistance was examined in bacterial assemblages from streams and ponds on the US Department of Energy's Savannah River Site (SRS) in South Carolina. Sites sampled have been impacted to varying degrees by contamination with organic compounds, heavy metals, and radioactive materials because of production of nuclear materials on the site. Antibiotic resistance in the culturable portion of the bacterial assemblage was determined from coloby formation on media containing antibiotics. Eight antibiotics, chloramphenicol, cycloserine, kanamycin, neomycin, novobiocin, rifampicin, streptomycin, and tetracycline, were used at concentrations of 50 and 200 μg ml−1. Statistically significant differences in frequency of antibiotic resistance were observed among sites and among dates at a single site. Bacterial densities (total and culturable), dissolved organic carbon (DOC) concentration, and human impact also varied among sites but bore no overall relationship to resistance frequency. SRS operations did not have a detectable impact on antibiotic resistance.
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