Abstract
We have analyzed staged human placentas by Northern, dot blot, and in situ hybridization to human c- myc probes. Placental RNA exhibits a stage-specific appearance of a 2.4 kb transcript of the c- myc gene. The frequency of this transcript varies 20 to 30 fold over the course of placental development, showing a peak at 4–5 weeks after conception, where the myc transcripts comprise about 0.05% by weight of the total placental mRNA. A clear decline in placental c- myc transcription is seen before the end of the first trimester of pregnancy. In situ hybridization to 125I-labeled myc probes demonstrates an unequal spatial distribution of myc transcripts in placenta with particularly high expression in the cytotrophoblastic shell of early placenta. Labeling of placental explants with 3H-thymidine, the localization of myc transcripts to cytotrophoblasts, and the temporal pattern of myc expression all support a strong correlation between myc transcript abundance and cytotrophoblast proliferation. We argue for a role for the c- myc gene in the proliferation of normal cells in this tissue.
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