Abstract
The combined use of Mag-indo-1 probe and laser confocal UV-microspectrofluorometry allowed us to investigate the spatial and temporal dynamic changes of the Mg2+variations in human tracheal gland (HTG) cells at the single cell level. Stimulation of HTG cells with either bradykinin, ouabain or extracellular high Mg2+concentrations (up to 10 mM) induced increases in intracellular Mg2+concentration [Mg2+]i. From a cytosolic basal concentration of 0.8 ± 0.3 mM in a medium free of Mg2+, an increase in extracellular Mg2+concentration from 1 to 10 mM, increased cytosolic [Mg2+]ifrom 1.4 ± 0.6 to 1.8 ± 0.8 mM after 10 min (p<0.05). We also demonstrated using line-scanned spectral images within single cells, that the [Mg2+]iis distributed uniformally in the nucleoplasm, but in contrast, showed marked local differences among different cytoplasmic regions, thus suggesting a functional heterogeneity in the intracellular Mg2+stores involved. The influx pathway for Mg2+in HTG cells was not inhibited by verapamil and appeared to be independent of [Ca2+]i.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
